TOPIC 382 - INTEGRATED IMAGING AND MOLECULAR ANALYSIS OF GLIOBLASTOMA USING SCOPE SEQ ON THE AIR FLOW

Combining both genomic analysis and highEresolution imaging on a cellEbyEcell basis typically relies on customized technology which often requires significant expertise and capital costCell MicrosystemsInchas partnered with Peter SimsPhD of Columbia University to commercialize a novel workflow for integrated imaging and sample preparation for single cell transcriptomic analysisDrSims workflowSingle Cell Optical Phenotyping and Expression SequencingSCOPEESeqwill be automated on a new microfluidic instrument under development by Cell Microsystemsthe AIREFLOWThe system provides detailed image analysis of single cellsin addition to fullyEautomated sample preparation of single cell transcriptomic analysisi escERNAESeqHerewe will apply this collaboratively developed platform to investigating the intractable cancerglioblastomaThe Phase I program will use glioblastoma neurospheres as a model system to validate the system s capabilities forfunctional dye imagingsingle cell molecular analysis and linking these data modalities on a cellEbyEcell basisDuring the proposed Phase II these capabilities will be further tested in patientEderived biopsies to test the tumor cell specificity of fluorescent dyes used during surgical brain tumor resectionWe expect the resulting system to have significant impact in this fieldas well as considerable commercial potential in the broader market