NEUROBIOLOGY OF LUTEIN

Several antioxidants exist. Many of these play an important role in various diseases, including neurological diseases e.g. Parkinsonism. We propose to characterize and study the role of a novel antioxidant-lutein. Neurobiological, neurochemical and biochemical techniques will be employed. $ = TOTAL AWARD AMTS & NOT LIMITED TO PORTION OF PROJECT RELATED TO SUBJECT OF SEARCH SUBPROJECT $ = TOTAL AWARD AMOUNT DIVIDED BY NUMBER OF SUBPROJECTS SOURCE: CRISP FORMAT F FY 97 LAST UPDATE 04-07-98 1QUERY 1536 ID SEARCH 06/01/98 PAGE 335 --PROJECT NUMBER......1 R43 GM55476-01 INVESTIGATOR NAME/ADDRESS FY 97 HADADI, M IRG/INTRAMURAL UNIT..ZRG1 NATURAL PRODUCTS RESEARCH INC AWARD AMOUNT......... $97,800 111 BOOKHAM LANE GAITHERSBURG MD 20877 PERFORMING ORGANIZATION: NATURAL PRODUCTS RESEARCH, INC. TITLE NEUROBIOLOGY OF ANTIOXIDANTS FROM PLANTS ABSTRACT: DESCRIPTION (Adapted from Investigator's Abstract): In this proposal the applicants will (1) prepare antioxidant-containing concentrates of mixed plant products, using HPLC and TLC and (2) assay the total and individual antioxidants and look for synergism between mixtures. $ = TOTAL AWARD AMTS & NOT LIMITED TO PORTION OF PROJECT RELATED TO SUBJECT OF SEARCH SUBPROJECT $ = TOTAL AWARD AMOUNT DIVIDED BY NUMBER OF SUBPROJECTS SOURCE: CRISP FORMAT F FY 97 LAST UPDATE 04-07-98 1QUERY 1536 ID SEARCH 06/01/98 PAGE 336 --PROJECT NUMBER......1 R43 GM55485-01 INVESTIGATOR NAME/ADDRESS FY 97 KOZYAVKIN, SERGEI A IRG/INTRAMURAL UNIT..ZRG2 FIDELITY SYSTEMS, INC AWARD AMOUNT......... $100,000 7961 CESSNA AVENUE GAITHERSBURG, MD 20879 PERFORMING ORGANIZATION: FIDELITY SYSTEMS, INC. TITLE THERMOFIDELASE TM FOR DNA SEQUENCING ABSTRACT: DESCRIPTION: (Adapted from the Applicant's Abstract). This proposal centers around the development of a commercial kit for DNA sequencing applications. The kit will contain a hyperstable protein that has been characterized by the applicant and determined to eliminate the majority of sequencing artifacts by its inclusion in sequencing reactions. Phase I consists of a feasibility study to formulate the kit for cycle sequencing reactions, including optimization of components and protocols. Phase II will include testing of the kit on the most difficult templates, optimization for fully automated sequencing projects and optimization aimed at diagnostic DNA sequencing applications. $ = TOTAL AWARD AMTS & NOT LIMITED TO PORTION OF PROJECT RELATED TO SUBJECT OF SEARCH SUBPROJECT $ = TOTAL AWARD AMOUNT DIVIDED BY NUMBER OF SUBPROJECTS SOURCE: CRISP FORMAT F FY 97 LAST UPDATE 04-07-98 1QUERY 1536 ID SEARCH 06/01/98 PAGE 337 --PROJECT NUMBER......1 R43 GM56035-01 INVESTIGATOR NAME/ADDRESS FY 97 HENDERSON, ERIC IRG/INTRAMURAL UNIT..ZRG2 BIOFORCE LABORATORY INC AWARD AMOUNT......... $100,000 2807 TORREY PINES RD AMES, IA 50014 PERFORMING ORGANIZATION: BIOFORCE LABORATORY TITLE MOFIFICATION OF ATOMIC FORCE MICROSCOPE TIPS ABSTRACT: The atomic force microscope (AFM) is an ultra-sensitive force transduction instrument capable of detecting force interactions between materials, molecules and chemical groups. Molecular force detection has application in biomedical diagnostics, drug development, genome analyses, and many other areas. Key to the implementation of molecular force detection int he commercial environment is the development of robust reliable modification AFM tips. Since specificity and sensitivity is dependent upon tip/sample contact area, it is critical to have access to the full spectrum of tip sizes for each molecular force detection application. In this proposal we will test a new method for the modification of AFM tips with functionalized microparticiles in a size range that fill s an existing void in modified tip sizes ( about 200nm to e(mu) in diameter). The method to be tested uses a near infrared diode laser to manipulate microparticles (in conjunction with mechanical manipulation) and locally "weld" them to AFM tips. Advantages of this approach include: tip modification in physiological solution, production of small diameter tips with define chemical and/or biological function, increased throughput, improved quality control, and ready development of semi-automated tip manufacture processess.