SYNTHESIS OF A SCA PROTEIN WITH ENZYMATIC ACTIVITY

A SINGLE-CHAIN ANTIBODY (SCA) PROTEIN WITH CATALYTIC ACTIVITY WILL BE PREPARED. THE SCA PROTEIN WILL BE DESIGNEDFROM THE VARIABLE REGION DEQUENCES OF ONE OF TWO ANTIBODIES FOUND TO HAVE LIPASE ACTIVITY, EITHER 2H6 OR 21H3. A GENE TO SYNTHESIZE THIS PROTEIN WILL BE CONSTRUCTED, AND THE PROTEIN WILL BE EXPRESSED IN ESCHERICHIA COLI. THE PROTEIN WILL BE RENATURED, PURIFIED, AND ASSAYED FOR ITS ENZYMATIC ACTIVITY. THIS SCA PROTEIN WILL BE ENGINEERED TO HAVE INCREASED STABILITY AND INCREASED CATALYTIC ACTIVITY DURING PHASE II OF THE PROJECT. THE SCA PROTEIN WITH CATALYTIC ACTIVITY PROVIDES THE MEANS TO ACCOMPLISH GENETIC CHANGES AND DETERMINE THE EFFECT ON CATALYTIC ACTIVITY AND STABILITY.