SBIR/STTR Research Award

Overexpression of Xenobiotic Metabolizing Enzymes

Award identifier ded614a82728ee6c1fd5f32e11573fe812f3fbc354c41ec5f76cc80539d9128bAwarding agency Department of Health and Human Services

Award amount

$455,626

Program and phase

SBIR · Phase II

Award year

1997

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Award details

Awarding agency
Department of Health and Human Services
Branch
Not reported
Recipient company
GENTEST CORPORATION
Proposal award date
Not reported
Contract end date
Not reported
UEI
Not reported
Solicitation number
Not reported
Topic code
Not reported

Research abstract

The plan is to develop and optimize a method for high level production of functional, catalyticallycytochromes P450 using baculovirus expression in insect cells. Cytochromes P450 are the principal enmetabolism of many drugs, protoxins, and environmental pollutants. This proposed method includes enhcytochrome P450 catalytic activity through the co-expression of cytochrome P450:NADPH oxidoreductasecytochrome b5. Human cytochromes P4502A6, P4502E1, and P4503A4 will be used as prototype enzymes fordevelopment. Catalytic constants, apparent Km, and turnover number will be measured for the optimizeexpressed enzymes and compared to the same enzyme isolated from human liver or produced using otherexpression systems. The eventual goal is to develop a complete panel of baculovirus expressed humanP450 isoforms. The commercial applications of this system would be for the study of human xenobiotic

Technology keywords

Not reported

Agency intelligence

Department of Health and Human Services

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