Picogram Non-Specific DNA Detection System

Based on preliminary results, construction of a general system for detection of DNA at thepicogram (1 x 10(-12)) level may be possible. The system would work in most aqueous solutionswithout regard to the sequence of the DNA. As proposed, it does not require sophisticatedinstrumentation and should be able to detect DNA in samples for a reagent cost of about 50 cents perassay.In particular, the system will be able to detect DNA in the presence of large amounts of proteins,carbohydrates, and small molecules. Components of the system are commercially available. The keyto making a commercially viable system is validation of the assay technique under conditions likely tobe of interest and benefit to potential customers, people doing laboratory research and pharmaceuticalproduction. A simple and reliable method of quantitating DNA concentration in complex mixtures isneeded. Without purifying DNA, no sequence independent method is available.