TOPIC 359: A DEVICE PROTOTYPE FOR ISOLATION OF MELANOMA EXOSOMES FOR DIAGNOSTICS AND TREATMENT MONITORING

The proposed work aims to develop a pre commercial prototype of an exosome isolation device to be used in clinical diagnostic laboratoriesWe will advance the device to facilitate exosome isolation for downstream liquid biopsy tests requiring analysis of proteinDNAor microRNAwhich rely upon acquisition of intact and pure exosomesThe device strategy will be designed to isolate total exosomes and then to separate discrete populations of tumor derived exosomesTEXand non malignant exosomesnon TEXThe device strategy will be advanced for melanoma exosome isolation in the proposed experiments but is expected to be applicable for other cancersTo achieve this study s objectiveswe will develop and optimize a filtration system incorporating the GNA Hemopurifier for isolation of total exosomes from biofluidsSpecific AimThe capabilities of the GNA Hemopurifier for isolating melanoma TEX and non TEX from diverse biofluids will be characterized by evaluating exosome depletion performanceSpecific AimWe will then construct a novel devicethe CSPGcartridgefor the isolation of TEX from total exosomesfollowed by testing the final workflow from the starting biofluid sample to the final TEX isolationSpecific AimSuccessful completion of this project will produce an exosome isolation system that will be applicable for high throughput exosome isolation and for TEX and non TEX separation from diverse biofluids of varying complexity in a clinical care setting in oncology