DEVELOPMENT OF NEW LATEX-BASED FLUORESCENT REAGENTS

WE WILL DEVELOP APPLICATIONS IN BASIC AND APPLIED RESEARCH AND IN MEDICAL DIAGNOSTICS FOR THE FLUORESCENT LATEX MICROSPHERES THAT HAVE BEEN PREPARED. THE SUPERIORITY OF THESE PRODUCTS IN TERMS OF THEIR RANGE OF SIZE, WAVELENGTH AND FLUORESCENCE INTENSITY WILL ENHANCE THEIR APPLICATION IN AREAS OF RESEARCH AND INDUSTRY THAT HAVE SENSITIVITY REQUIREMENTS THAT CURRENTLYAVAILABLE FLUORESCENT LATEX AND OTHER FLUORESCENT REAGENTS CANNOT MEET. THE NEW MICROSPHERES WILL BE PREPARED FROM OIL-SOLUBLE FLUORESCENT DYES RECENTLY DEVELOPED BY OUR RESEARCH. THE SPECIFIC APPLICATIONS ARE: (1) NEW FLUORESCENT LATEX-BASED HYBRIDIZATION REAGENTS, (2) LABELINGREAGENTS WITH IMPROVED FLUORESCENCE INTENSITY, GREATER STOKES SHIFTS AND MULTIPLE EXCITATION/ EMISSION FOR BETTER DETECTABILITY AND MULTI-COLOR APPLICATIONS IN IMAGING AND FLOW CYTOMETRY, AND (3) NEW, ULTRA-SENSITIVE FLUORESCENT PROBES FOR LOW-DENSITY CELLULAR RECEPTORS.