HIGH-RESOLUTION, TWO-DIMENSIONAL ELECTROPHORESIS ALLOWS A LARGE FRACTION OF THE ESTIMATED 30,000 TO 50,000 HUMAN PROTEINS TO BE IDENTIFIED BY MAP POSITION AND TO BE QUANTITATED.

HIGH-RESOLUTION, TWO-DIMENSIONAL ELECTROPHORESIS ALLOWS A LARGE FRACTION OF THE ESTIMATED 30,000 TO 50,000 HUMAN PROTEINS TO BE IDENTIFIED BY MAP POSITION AND TO BE QUANTITATED. NORMAL AND CANCER CELLS HAVE BEEN MAPPED IN ORIENTING STUDIES AND MANY DIFFERENCES FOUND. TO GIVE MEANING TO THSES RESULTS, AND TO SYSTEMATICALLY EXPLOIT THEM, A LARGE DATABASE (THE CANCER PROTEIN DATABASE) INCLUDING ALL AVAILABLE DATA ON EACH SPOT IS REQUIRED, WITH THE DATABASE DIRECTLY LINKED TO CRT MAP DISPLAYS. THE DATABASE WILL INCLUDE (FOR EACH PROTEIN) INTRACELLULAR LOCATION, TYPES OF CELLS IN WHICH FOUND, NAMES, FUNCTIONS, DISEASE CORRELATION, BIOPHYSICAL DATA, LITERATURE CITATIONS,MEMBERSHIP IN COREGULATED SETS, DIFFERENTIATIONAL STAGE OF OCCURRENCE, AND CHROMOSOMAL LOCATION OF THE CODING GENE. THE THESES TO BE EXPLORED ARE THAT CELLS ARE HIGHLY INTEGRATED SYSTEMS TO BE UNDERSTOOD IN TERMS OF PROTEIN SETSAND THE REGULATIONAL CIRCUITS INVOLVING THEM, AND THAT COMPOSITIONAL CHANGES IN CANCER CELLS ARE NOT RANDOM, BUT REFLECT ALTERATIONS IN NORMAL REGULATIONAL AND DIFFERENTIA- TIONAL FUNCTIONS. THIS PHASE I PROJECT IS FOR THE CONTINUEDDEVELOPMENT OF THE TYCHO AND KEPLER DATABASE SYSTEMS, SPECIFICALLY DIRECTED TO CANCER STUDIES, IN A PRIVATE SETTING.