A STANDARD REPOSITORY FOR HUMAN GENE MAPPING

THIS PROJECT WILL ESTABLISH A SERIES OF PRODUCTS TO FACILITATE THE GENERATION OF HUMAN GENETIC MAPS BY PREPARINGTRANSFORMED LYMPHOCYTES AND EXTENSIVE DATA PROFILES FROM MEMBERS OF ORIENTAL AND CAUCASIAN FAMILIES. THE TEST PANEL WILL BE OF SUFFICIENT SIZE SO THAT RESEARCHERS CAN: (1) DETECT EVEN RARE RESTRICTION FRAGMENT-LENGTH POLYMORPHISMS (RFLP'S), (2) DETERMINE THE FREQUENCY DISTRIBUTION AND HARDY-WEINBERG EQUILIBRIUM OF THESE RFLP'S IN ORIENTALS AND CAUCASIANS, AND (3) COMPARE THE GENETIC MAPS OF CAUCASIANS AND ORIENTALS AT THE LEVEL OF 5-PERCENT RECOMBINATION. TO PROVE THE UTILITY OF THIS REPOSITORY AND GENOTYPES OF THESE POPULATIONS FOR SPECIFIC CANCER-RELATED GENES, THE DNAFROM 320 INDIVIDUALS WILL BE PURIFIED AND DIGESTED WITH EACH OF EIGHT RESTRICTION ENZYMES, AND FORMATTED INTO SPECIFIC SETS OF DNA BLOTS. THESE DNA BLOTS WILL BE SCREENED FOR RFLP'S USING DNA PROBES REPRESENTATIVE OF PROTO-ONCOGENES, ANTI-ONCOGENES, AND P450 GENES. RESULTS WILL BE ENTERED INTO THE COMPUTER ALONG WITH THE INDIVIDUAL DATA PROFILES. THESE STUDIES WILL SHOW THAT THIS REPOSITORY CAN BE ACCESSEDEFFICIENTLY VIA DNA BLOTS, THAT ONE CENTRAL LABORATORY CAN PREPARE DNA BLOTS AT A LEVEL OF REPRODUCIBILITY HIGHER THAN THAT OF INDIVIDUAL LABORATORIES, AND THAT SUCH A REPOSITORY WILL BE A STANDARD SOURCE OF DNA'S FOR EASILY GENERATING HIGH-DENSITY GENETIC MAPS.