Preparation of Vaginal Lactobacillus Suppository

We will develop a commercially available vaginal suppository containing a hydrogenperoxide-producing strain of Lactobacillus for use in colonization of the vagina. In this project, a strainof Lactobacillus will be identified based on adherence to vaginal epithelial cells, hydrogen peroxideproduction, and growth characteristics. Methods for strain preservation and maintenance of viability,biochemical, physiologic and genetic characteristics during large scale production will be optimized. Theeffect of suppository delivery formats and components including various cream and gel bases, growthor nutritional additives on successful bacterial rejuvenation, adherence and hydrogen peroxide productionwill be investigated.