Development of 1 KB DNA Thermocycle Sequencing Kit

The objective of this application is to optimize and exploit a DNA thermocycle sequence kit which c1 kilo base of readable DNA sequence. As current PCR cycle sequencing kits give around 400 base paireach reaction, this innovation will at least double the amount of DNA sequence that can be read fromThe proposed kit will use common reagents and instruments, optimal PCR sequencing conditions, a gelsystem, and a new sequencing reaction strategy for increasing evenly the intensity of all bands on tanticipated that these innovations will make DNA sequencing more efficient and accurate.