Thermus Expression System

We will develop a regulated expression system for the extreme thermophile Thermus flavus. This systproduction of commercially important thermostable enzymes. In the Phase I feasibility study we willfrom Thermus, perform minimal structural analyses, and test their regulation using reporter genes inpurpose, we will develop an integrative promoter-probe shuttle vector for Thermus based on a promotegalactosidase and kanamycin phosphotransferase and a chromosomal integration system. Since commerciaproteins are often secretable, a search for signals suitable for regulated expression-secretion systII will focus on the development of the expression system into a functional tool for expressing bothThermus, and for model experiments in protein thermostabilization. Further study on expression of hyproteins is also planed. In Phase III the expression system will be applied to the development of coenzymes.