HIGH PERFORMANCE IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY

IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY (IMAC) HAS SUBTANTIAL UTILITY AS A PROTEIN PURIFICATION TOOL FOR BOTH ANALYTICAL AND PREPARATIVE APPLICATIONS. THERE IS A STRONG MARKET NEED FOR AN IMAC SUPPORT SUITABLE FOR HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC). RESEARCHERS ARE DEVELOPING AN HPLC SUPPORT WITH GOOD PH STABILITY, LOW NONSPECIFIC BINDING, GOOD CAPACITY, DURABILITY, AND RAPID ADSORPTION KINETICS. CAPTURE LIGANDS ARE IMMOBILIZED ON LARGE PARTICLE SILICA VIA A 50 A HYDROPHILIC TETHER. THIS RESEARCH COMBINES THE HPLC AFFINITY SUPPORT WITH WELL CHARACTERIZED AFFINITY PROBES FOR IMAC OF PROTEINS. RESEARCHERS ARE PREPARING AND CHARACTERIZING TWO NEW IMAC SUPPORTS FOR HPLC, MEASURING THE PROTEIN ADSORPTION CAPACITY FOR MODEL PROTEINS UNDER VARIOUS CONDITIONS, AND DETERMINING RESOLUTION OF CLOSELY RELATED PROTEIN ON THE COLUMNS. THESE STUDIES DEFINE THE UTILITY OF IMMOBILIZING IMAC AFFINITY PROBES ON HYDROPHILIC TETHERS FOR RAPID, HIGH RESOLUTION PROTEIN PURIFICATION.