MULTIPURPOSE CLONING/SUBCLONING SYSTEM

BIOTIX PROPOSES TO DEVELOP SYSTEMS FOR STABLY CLONING SMALL AND LARGE DNA FRAGMENTS THAT ARE ALSO OPTIMIZED FOR PRODUCING A SERIES OF OVERLAPPING SUBCLONES USING EITHER THESINGLE-STRANDED OR THE DOUBLE-STRANDED PROCEDURE. THE VECTORS WILL BE CONSTRUCTED FROM EXISTING VECTORS UTILIZING A POWERFUL NEW INSERTION TECHNIQUE. SUCCESS IN THE CONSTRUCTION OF THESE VECTOR SYSTEMS WILL LEAD TO THE DEVELOPMENT AND PRODUCTION OF A SERIES OF INTEGRATED KITS DURING PHASE II. THE SPECIFIC PHASE I OBJECTIVES ARE: (1) CONSTRUCTION OF A VECTOR WITH A MULTIPLE CLONING REGION OPTIMIZED FOR BOTH SINGLE- AND DOUBLE-STRANDED SUBCLONING PROCEDURES; AND (2) MODIFICATION OF THE VECTOR TO INCLUDE A COS SITE TO PERMIT UP TO 40 KB FRAGMENTS TO BE STABLY CLONED.