Multi-omics profiling of individual exosomes for origin-tracing, biomarker discovery, and biological function characterization

PROJECT SUMMARY / ABSTRACT Extracellular vesicles (EVs) exhibit high heterogeneity in biofluids, a feature that traditional bulk-level analysis approaches fail to capture in terms of individual variations. While various techniques exist for single-EV analysis, the majority focus primarily on surface proteins. Transcriptional analysis at the level of individual EVs, however, remains largely unexplored. To bridge this gap, we propose the development of a platform for multimodal digital profiling of individual EVs, leveraging next-generation sequencing and an optimized system for multiplex library preparation. This proposed platform will serve as a unique tool for high-throughput absolute quantification of single-EV genes and proteins. It aims to offer high-sensitivity, multi-dimensional biological insights, thereby potentially accelerating the advancement of EV-based diagnostics and targeted therapies.