On-Orbit Solution Recycling Methods to Improve Scale of Layer-by-Layer Artificial Retina Manufacturing

LambdaVision has developed a protein-based artificial retina to restore vision to the millions of people blinded by retinal degenerative diseases, including retinitis pigmentosa and age-related macular degeneration. The artificial retina thin films are manufactured using a layer-by-layer (LBL) assembly technique, in which alternating layers of the light-activated protein, bacteriorhodopsin, and a polycation binder are deposited onto an ion-permeable film. LambdaVision is leveraging the unique properties of microgravity that allow for more ordered and consistent three-dimensional assembly of the protein and polymer layers. The microgravity environment has the potential to improve the quality of the films, reduce defects, and enhance the stability and performance of the artificial retinas for future preclinical and clinical trials. In collaboration with Space Tango, LambdaVision has completed a series of proof-of-principle microgravity experiments that have established a foundation for producing artificial retinas using a low-Earth orbit (LEO) platform. This effort led to the optimization of a fluidic chamber LBL manufacturing device, though further steps are required to improve the scale for commercial production. The Phase I research strategy involved enhancing the precision of quantification methods for polyion solutions, developing effective filtration methods, optimizing UV-C sterilization for bioburden reduction, and establishing in situ analytical tools to assess solution quality. Building on these results, this Phase II work plan will integrate these advancements into a closed-loop system for efficient, sustainable LBL manufacturing that will improve scale, reduce up-mass raw materials, and reduce waste. The deliverables will add new on-orbit tools to the established LBL manufacturing platform, and solution recycling techniques will ensure that artificial retina production can be achieved at an increased scale on the ISS or future commercial LEO destination.