Commercialization of methods and reagents for high-throughput single cell analysis

PROJECT SUMMARY / ABSTRACT Single-cell RNA sequencing (scRNAseq) provides unprecedented insight into the gene regulatory pathways of living cells in complex tissues. Fluent BioSciences has developed Pretemplated Instant Partitioning for single-cell RNA sequencing (PIPseq) that eliminates the cost and complexity of bespoke microfluidics and instrumentation and features a simple, flexible workflow that can be implemented in any laboratory setting. With Phase I SBIR support (1 R43 GM137648-01A1) we have demonstrated that PIPseq is intrinsically scalable and is capable of capturing from hundreds to millions of cells in a single reaction. The advent of new, high capacity, and low cost sequencing modalities such as that developed by Ultima Genomics makes high cell-input single cell transcriptomics not only feasible but practical, enabling previously inaccessible experimental modalities. In this proposal, we will expand the capabilities of PIPseq single cell analysis to provide simple, scalable, and cost-effective solutions for high cell input applications including deep tissue profiling, multiplexed drug testing, and pooled CRISPR screening.