Novel probes for real time monitoring of dissolved gases and their isotopologues in aquatic ecosystems

Ocean ecosystems are critical to the regulation of Earth’s climate and biodiversity, while also hosting a range of direct and indirect benefits to people, from being a food source to recreation. Coastal ocean systems are dynamic regions especially rich in diverse biological and geochemical interactions. However, major gaps exist in our knowledge of the primary biogeochemical processes and the factors regulating their relative importance. Nitrous oxide (N2O) and methane (CH4), produced and cycled within coastal and ocean environments, are important greenhouse gases with major roles in climate change. Our understanding of the distribution, dynamics, and forcers of the underlying processes controlling their fluxes is limited by a lack of high-resolution spatial-temporal measurements. The overall objective of this project is to design a field deployable, real-time, in situ system to quantify dissolved greenhouse gases (N2O and CH4) and their isotopologues in ocean ecosystems. In Phase II we will build permeable, hydrophobic probes to extract dissolved gases without intrusion of liquid water; multiplex an array of probes with a field-ready, efficient sample collection and transfer scheme; and interface the probe array and sampling system with high-sensitivity infrared spectrometry. We will demonstrate the field-ready system with deployments at several coastal ecosystems.