Development of a high precision and high order DNA counting platform

PROJECT SUMMARY/ABSTRACT Enumerix has developed a cost-effective method to perform high order DNA counting at high precision called direct count PCR (dcPCR). dcPCR uses a spin column method that generates millions of partitions directly into PCR tubes for target amplification, using standard laboratory centrifuge and thermal cyclers, followed by an ultra-rapid method to count up to 1 million copies of DNA directly from the same PCR tube. We have demonstrated that our technology far exceeds the precision of digital PCR, and can perform at a DNA counting range akin to quantitative PCR and next generation sequencing, which makes it a suitable next-generation DNA counting platform. For this proposed Phase II project we plan to further develop our dcPCR platform with the goal to distribute the technology to end-users to replace quantitative PCR and digital PCR.