Developing Cultivation Methods and Characterization of Superior Biological Compounds for Neotropical Ganoderma Species

Huerto Rico LLC is an agricultural startup located in Puerto Rico that specializes in theproduction of gourmet mushrooms for the local restaurant market with a special emphasis onresearch and development of new strains based on understudied tropical mushroom species. Theteam has identified six native species of Ganoderma spp.; one of which species has never beendescribed in literature before. Reishi is the common name for mushrooms of the genusGanoderma and is known as the 'mushroom of immortality' in Asia where it is documented tohave been consumed for millennia (Hapuarachchi et al 2018 Azizi et al 2012). AlthoughGanoderma-based products are widely used worldwide natural supplements are a poorlyregulated industry and several species are sold under the name "Reishi" (Zhou et al. 2011Hobbs 2017 Hapuarachchi et al 2018 Wu et al. 2017). The team's preliminary results show thatnot all Reishi are created equal and that knowing which species are being grown and theirchemical profile is important when making claims about the commercial and biological benefitsof these productsThe goals for this Phase I project are to: 1) develop improved cultivation methods by assessingsuitable substrates for cultivation of Ganoderma tuberculosum Murrill Ganoderma aff.multipileum Ding Hou Ganoderma multiplicatum (Mont.) Pat. Ganoderma martinicense Welti& Courtec. Ganoderma subamboinense Henn. and the newly discovered endemic subgenus ofGanoderma puertorricencis. 2) Evaluate the biochemical profile and phytochemical content ofeach species and compare them to that of Ganoderma lucidum to assess their biological activity(e.g. antioxidant levels anticancer properties) and identify novel chemical structures that mayincrease the effectiveness and overall quality of Ganoderma natural products. The long-term goalof this proposal is to create a new agricultural product with improved biological properties basedon neotropical Ganoderma subspecies which will help improve the overall quality ofGanoderma-based products grown and manufactured in the United States.