SBIR/STTR Research Award
Rapid field testing of field water samples for total coliform and E. coli using isothermal amplification and unique micron-sized particles for cell capture and concentration
Award amount
$999,285Program and phase
SBIR · Phase IIAward year
2020Award details
- Awarding agency
- Department of Defense
- Branch
- Defense Health Agency
- Recipient company
- IFYBER LLC
- Proposal award date
- 2020-09-24
- Contract end date
- 2023-01-23
- UEI
- SWJHU7YPRAU9
- Solicitation number
- 17.1
- Topic code
- DHA17-004
Research abstract
iFyberLLC proposes to develop acoliform assay with differential detection of E.coliand total coliform that will circumvent thecurrent challenges with detectingthese organismsatalevel of1 CFU per100mLin less than 8 hours. The proposed assay targetsaresult in less than 8 hours by making use of novel microspherereagents forcaptureand concentration of thetargetcells,and couplingthesereagents with loopmediated amplification (LAMP) ofcoliform and E.coligeneticmarkers with fluorescence detection.Microspheres will be used to concentratethe targetcells byat least1000fold,wherethey will then befurtherenriched with growth medium,and their respectivegenetictargets will be subsequentlyamplified through LAMP. This PhaseIIeffort is building offasuccessful PhaseI project that showed feasibility for theconcept. PhaseII will befocused on assay optimization,kit development,and development ofaalpha prototypesystem that will bevalidated next to currentlyapproved EPA ATP methods.
Technology keywords
Not reported
Other awards by this recipient
Verified by the same UEI. These are other public SBIR/STTR award pages, not a merged company record.
- Advancement of a Cu-CMC nonwoven dressing for burns and expansion of the Cu-CMC platform
- Standard-issue Antimicrobial Combat Burn Gauze
- Development of wound care formulations for PVP/NO: A novel nitric oxide releasing platform with potent antimicrobial and anti-biofilm properties
- Development of Novel Broad Spectrum Antimicrobial Corneal Tissue Storage Solutions
- Peptide based wound dressing for prevention and management of infected wounds
Awards with the same topic code
Verified by an exact topic code match. This is not a text-similarity match.
- Field portable Coliform bacteria & E. Coli RNA biomarker LAMP - OSD system
- Rapid field testing of field water samples for total coliform and E. coli using isothermal amplification and unique micro-sized particles for cell capture and concentration
- Rapid, Portable Detection of Coliforms and E. coli. in Drinking Water
- Field portable Coliform Bacteria and Escherichia Coli RNA expression LAMP-OSD detection system
- A Device to Rapidly Detect Coliform Bacteria and Escherichia Coli in Field Water Samples
Research funding history
16 SBIR/STTR records share this UEI (2009–2025). Reported award amounts total $6,586,065. Reported amounts are not federal obligations.
Phases: Phase I, Phase II, Phase II – Direct to Phase II
Agencies: Department of Defense, Department of Health and Human Services, National Science Foundation