Development of High Squalene Tobacco

SynShark LLC aims to create an economically viable source of squalene from American tobacco.Squalene is a triterpene important to the cosmetic and pharmaceutical industry. Unfortunatelysqualene is mainly sourced through the "livering" of millions of deep-sea sharks threatening theirpopulations. Through the Company's exclusively licensed technology for triterpene squalenewhich is extracted from metabolically engineered tobacco (Nicotiana tabacum L. 1068) SynSharkdemonstrated that it can express high squalene levels through a novel photosynthetic method.Specifically the Company showed a capacity to shift carbon flux into the MEP pathway allowingfor greater accumulation of tobacco squalene. During Phase II the Company will optimize thepreviously studied method for increased terpene production and storage within SynShark tobacco.Because the cropping methods and postharvest infrastructure are already in place due to tobacco'shistorical importance in agriculture and the American economy tobacco can be easilycommercialized. Tobacco offers an ideal synthesis platform for modification for commercialapplications due to high transformation efficiency. SynShark has previously produced stablesqualene producing tobacco varieties at pilot scale and processed fractioned bioproduct for themarket. Phase II research will focus on optimizing this method and creating a quantification of theportfolio of high quality ingredients being expressed in SynShark's tobacco beyond squalene. Theknow-how developed to bring squalene to market will support a rapid go-to-market scenariobeyond Phase II. Through the optimizing of squalene extraction from SynShark tobacco we plan toexpand our platform of natural products to satisfy the highly demanded market.