Functional drug fingerprinting with all-optical electrophysiology

Project Summary Functional drug fingerprinting with all-optical electrophysiology The Optopatch platform recently developed at Q-State Biosciences, which uses engineered optogenetic proteins, custom microscopes, and software, makes it possible to simultaneously stimulate (blue light) and record (red light) electrical activity from ~100 neurons with 1 millisecond temporal resolution, single-cell spatial resolution and high signal-to-noise ratio. The Optopatch assays can record hundreds of parameters characterizing intrinsic excitability and neuronal function in both rodent and human induced pluripotent stem cell-derived neurons. Using these tools, we propose to develop a “drug fingerprint” database, where we characterize the impact of a library of drugs and tool compounds on neuronal excitability. The database will capture the unique functional effects of each compound and represent them as vectors in a reduced dimensionality fingerprinting space. The database can be leveraged in several ways in both internal programs and with pharma partners:1) Identification of targets of hits from a phenotypic screen. – Phenotypic screening offers theadvantage of testing compounds in a disease-relevant cell type or animal model but has thedisadvantage of not knowing the target(s) of effect. Target ID is essential information for de-risking the drug discovery process and matching the hits’ functional effects to the drugfingerprint database can accelerate or augment standard target identification approaches.2) Rapid identification of therapies in personalized medicine. – Q-State is expanding capabilitiesto identify therapeutic approaches for patients with severe genetic disorders who have a shortlife expectancy. Optopatch has identified functional deficits in neurons derived from individualpatients relative to healthy controls, and we hope the drug fingerprint database can be usedto expedite and focus therapy selection to those with high potential to correct the problem.This obviates the need to screen and entire drug library in patient neurons; requiring only themost promising compounds to be tested and saving time for a patient where time is limited. In this proposal, we first assemble the library and build the drug fingerprint database. We then test the two applications. Fidelity of target identification is tested using compounds with known targets, and the ability to identify phenotype-reversing drugs is tested using cell lines and phenotypes already established at Q-State. The tools developed will be a permanent asset and should catalyze the development of new therapeutics.Project Narrative Functional drug fingerprinting with all-optical electrophysiology Q-State has developed engineered proteins and custom microscopes that enable us to rapidly record electrical signaling in thousands of neurons grown in a dish and profile how their behavior changes in response to an applied drug. By testing a large set of drugs and tool compounds with known mechanism of actions in neuron, we will build a database of analytically characterized drug responses called “fingerprints.” We will use this fingerprint database to rapidly identify drugs that 1) might correct the aberrant behavior of neurons derived from a patient with a neurological disorder or 2) help identify the molecular target of a compound of interest discovered in a drug screen.