Automation and Performance-Based Selection to Improve the Reproducibility of MSCs

Project Summary The purpose of this proposal is to advance the field of Mesenchymal Stromal CellMSCbiology and particularly the process of translating MSCs into safe and effective therapiesThe Broad Aim of this proposal is to demonstrate that the Cell Xautomation platform enables users to generate culture expanded humanmesenchymal stem stromal cellpopulationshMSCswith greater quality and with less variation from batch to batchSuccess will open the valued market of MSC generation and commercial fabrication to Cell XTechnologiesThe Cell XDevice is the core product of Cell X Technologieswhich was founded inas a spin off company derived from technology generated in the Muschler Lab at the Cleveland Clinic with engineering support from Parker Hannifin CorporationCell X combines four essential automated featuresRapid large field of viewLFOVGoogle Earthwhole wellimage analysisenabling the user toquantitatively capture and useperformance basedcolonycloneattributes for cell source selectionPrecise automated methods for biopsypicking or weedingremovalof colonies with specific attributesAutomated methods for removal and replacement of media during cell expansioni emedia changeConsistent and standardized documentation of parameters and images associated with each interventionhMSC variation represents a profound point of ongoingpain pointin the biomedical research community and in the emerging industry of regenerative medicine and cellular therapiesClinical use demands high levels of repeatability and reproducibilityDonor to donor and batch to batch variation also confounds the utility of culture expanded MSCs in drug screeningtoxicity testingevaluation of biomaterialsetcDemonstration of the value of Cell Xautomation for optimizing MSC generation and fabrication will greatly advance the value and demand for Cell XPlatforms for researchdrug development and clinical fabrication Project Narrative This proposal seeks to advance the field of human Mesenchymal Stromal CellMSCbiology and particularly the process of translating MSCs into safe and effective therapiesWe will specifically determine if the Cell Xautomation platform enables users to generate MSCs with greater quality and batch to batch variation