High Performance Liquid Phase Ion Mobility Spectrometer for Omics Applications

Project Summary Abstract This Small Business Innovation Research Phase I project will demonstrate the feasibility of a novel approach for achieving unprecedented high levels of resolution in ion mobility spectrometryOur approach will not only improve the resolving power of an ion mobility spectrometer substantiallybut also enable efficient separation of ions without the activation of the internal energy of the ionswhich is an essential benefit to the study of many life sciences problemsFor examplethe proposed instrument would be an ideal tool for studying large protein complexes with folded structures vulnerable to even minor alterations from ion internal energy excitationT here is a hybrid between ion mobility spectrometry and electrophoresisinheriting the advantages of both methods while expanding the breadth of potential applicationsThe Phase I focus is on the development of a standalone system that will validate the resolving power and the effective ion temperature during ion mobility analysisCommercial adoption of this approach will push contemporary ion mobility spectrometry into new frontiers of chemical analysis and will considerably facilitate future applications ofomicsmethodsi eanalysis on the systems leveltowards clinical usehe innovation proposedProject Narrative The broader impact of this project is to provide greater capability of high resolution ion mobility spectrometry into anomicspipelinei eanalysis on the systems levelOmicsbased strategies are already a promising tool for the identification of diagnostic and prognostic biomarkers of disease