Single clone discovery using an image-based cell isolation platform

Under microscopeit is easy to identify cells of specific morphological phenotypes or interacting with other cell typesBut it is not trivial to pick the very cell out without submicron precision mechanical instrumentmicro pipetting or laser micro dissectionTo facilitate image based single cell isolationwe seek to develop an LCD aided selection under microscopeLASUMtechnology that will allow us to pick single live cells without bulky instrumentation or tedious single cell handling skillsAlsoas the capturing process is done by one single overall exposure stepthe throughput of this technology will be greatly enhanced compared with single cell manipulating technologiesThe proposed method can be used to select and isolate cells based on organelle translocationchromatin morphologyphagocytosissynapse formationprotein co localizationand most importantlycell cell interactionsAnd we will complete this proof of concept study by implementing LASUM to the following areasaphage enrichment on single cells or beadsbisolation of tumor killing immune cell clones Narrative Under microscopeit is easy to identify cells of specific morphological phenotypes or interacting with other cell typesBut it is not trivial to pick the very cell out without submicron precision mechanical instrumentmicro pipetting or laser micro dissectionTo facilitate image based single cell isolationwe seek to develop an LCD aided selection under microscopeLASUMtechnology that will allow us to pick single live cells without bulky instrumentation or tedious single cell handling skills