Rapid, Multiplexed Point-Of-Care Molecular Detection of Respiratory Pathogens without Sample Prep

RapidMultiplexed Point Of Care Molecular Detection of Respiratory Pathogens without Sample prep CrossLife TechnologiesIncandampUniversity of Washington RESEARCH andampRELATED Other Project InformationProject Summary Respiratory infections are one of the most common reasons for doctor visits and the most common illness resulting in missed work or schoolRespiratory infections are caused by viruses or bacteriaAcute respiratory infections such as pneumoniafluand respiratory syncytial virus are responsible formillion deaths worldwide each yearAcute respiratory infections are world s third leading cause of death worldwide mostly in the developing world where resources are limitedand labs or expensive instruments in labs are not availableRapid low cost tests for pathogens that cause respiratory infections are needed for situations where a lab or transport to a lab is not availableespecially in the resource limited settings of the developing world where the burden of acute respiratory infections is the highestWe aim to develop a rapid point of test that detectsrespiratory pathogensviruses and bacteriaand is appropriate for non laboratory settings and non technical personnelOur proprietary test exploits a novel probe reaction chemistry that allows multiplexed detection of DNA or RNA without sample purificationmaking it operable as a simplehandheld testPatient samples to be input directly into the device without pre processing and a disposable cartridge carries out amplification of all targets and reports visual results that can be read by eye or a low cost imagerThe key goals of the project are todevelop a prototype device that can detect up torespiratory pathogens and tovalidate it using clinical samplesAvailability of rapid turnaround respiratory pathogen testing will allow infected patients to be isolated with infection control and or treated in real time without loss to follow upwhich could avoid costs for hospital stays$a dayenable better outcomeand contribute reduced antibiotic resistanceFinallythe TARAPlex test will be less expensive$in the U Sthan BioFire multiplexed PCR$per testRapidMultiplexed Point Of Care Molecular Detection of Respiratory Pathogens without Sample prep CrossLife TechnologiesIncandampUniversity of Washington RESEARCH andampRELATED Other Project InformationNarrative Respiratory infections are one of the most common reasons for doctor visits and the most common illness resulting in missed work or schooland they are world s third leading cause of death worldwideWe will develop a rapid low cost test for respiratory infections that can be used for rapid diagnosis in the US medical facilities or low resource settings globally