Enhancing transgenic laboratory animal genotyping

SUMMARY ParaTechsmission is to develop innovative technologies and bring to market novel products to enable discovery and development in the area of biotechnologyOur expertise with laboratory animal models provides the basis for a new genotyping process for use in the biomedical industrystarting with aRs alternative for rodent genotypingUltimately our vision is to expand the technology to personalized genotyping so that an individual can test for the presence or absence of a gene in the privacy of their homeThis new system is called structured nucleotide nanoparticle advanced genotypingor SNNAGSNNAG is a novel analytical method based upon the properties of nucleic acid hybridization to detect specific gene sand ultra sensitive detection enabled by fluorescent mesoporous silica nanoparticlesmSNPsThis does not rely on existing techniquesPCR and DNA sequencingbut utilizes the selective properties of nucleic acid hybridization between tissue DNA samples and fluorescentoligonucleotide labeled mSNPs to support direct genotypic detectionThe SNNAG system will be coupled with a new system for oral sampling of tissue in rodentsThis novel devicethe LolliswabTMentices rodents to deposit tissue on the deviceeliminating the need for toetailor ear samples for genotypingGenotyping workflows entail tissue collectionDNA isolationsignal amplificationby PCR and or sequencingand detectionThe Lolliswab collection device and SNNAG detection system will provide fasteasyand more humane genotyping to laboratory animal researchThis novel system will provide an alternative to painful tissue sampling procedures in rodents and pair it with an easy and ultra sensitive detection systemOur shortterm project goal of improving laboratory animal genotyping is both a significant research and commercial goal and a key step toward validating and reaching the ultimate goal of this research and commercialization effortestablishing personalized genotype detection NarrativeThe genotyping market is undergoing explosive growth with annual increases in excess ofwith revenues ofB inand projected atB byHoweverthere are privacy concerns that are not well addressed by industry as well as opportunities to improve its speed and accessibilityOur long term goal is to develop a personalize genotyping system so that individuals can test for presence or absence of a specific gene in their homes!