Rodents Derived Synthetic Blood Brain Barrier Models for Chemical Toxicity Screening

AbstractThe overall goal of this study is to develop and demonstrate a novelpredictive in vitro organotypicD culture models of the blood brain barrierBBBderived from rodents for chemical toxicity screeningIn contrast with current Transwell based static and microfluidic assaysour commercially available SynBBB model enables real time visualization and quantitation of transport permeation under physiological microcirculatory size and flow conditionswhile simultaneously simplifying on chip and off chip analysisThe apical side provides aD architecture of endothelial cells while the basolateral side supportsD glial cell co culturesPhase I study seeks to demonstrate the feasibility of the SynBBB model for predicting central nervous system toxicity of essential and heavy metals specifically across the BBBIt will culminate with a clear demonstration of the model with validation against in vivo studiesDuring Phase IIwe will develop a higher throughput platformassayswith automated measurement of physiological end points for mechanistic understanding and detailed validation against animal studiesA multi disciplinaryindustry academic partnership including CFDRC and Albert Einstein College of Medicine encompassing expertise in microfluidics cell based assaysblood brain barrierchemical and heavy metal derived neurotoxicitydrug discovery and developmentand therapeutic evaluation will oversee the development of this modelThe developed assay will have critical applications in basic research for understanding of chemical toxicity mechanisms and development of toxin neutralization strategiesThe end product will be commercialized to government agenciespharmaceutical firmsdrug research labs and universities non profit centers engaged in chemical toxicity screeningdrug discoveryand drug delivery Project NarrativeThe overall goal of this study is to develop and demonstrate a novelpredictive in vitro organotypicD culture models of the blood brain barrierBBBderived from rodents for chemical toxicity screeningThe developed assay will have critical applications in basic research for understanding of chemical toxicity mechanisms and development of toxin neutralization strategiesThe end product will be commercialized to government agenciespharmaceutical firmsdrug research labs and universities non profit centers engaged in chemical toxicity screeningdrug discoveryand drug delivery