Micro-fluidic Photometer with Adaptive Mask

A flow micro-photometer is proposed that can measure absorption and backscatter from single aquatic particles including phytoplankton, detritus and minerals. This instrument will make use of a novel adaptive diaphragm to define an analysis region of interest that exactly matches the size, shape and orientation of each particle being analyzed. A micro-fluidic chip will be used to convey the particles in a sequential fashion through the analysis region of the system. The proposed instrument will be designed to be capable of measuring the transmitted and backreflected light of particles from 0.5 microns to 100 microns in diameter. Initially the tool will measure the optical properties at three wavelengths in the visible, 440 nm, 520 nm and 670 nm. The instrument will also provide images of each particle, from which the identification of the type of particle being analyzed is possible, as well as provide morphology information.