Development of A Novel Magnetic Exosomal Assay for Diagnosis of Diseases with Impaired Vascular Integrity

DESCRIPTION provided by applicant This Small Business Innovation Research Phase project aims to develop and characterize a novel Magnetic Exosomal Assay to isolate and detect single intact exosome concentrations in human serum This proposed research will also evaluate the diagnostic potential of the Magnetic Exosomal Assay for diseases with impaired vascular integrity Vascular integrity or the maintenance of blood vessel continuity is a fundamental process maintained through endothelial cell cell junctions Defects in endothelial barrier function are an initiating factor in several disease processes including atherosclerosis ischemia reperfusion tumor angiogenesis cancer metastasis diabetes sepsis and acute lung injury An initial event in impaired vascular integrity is a dramatic increase in a novel type of extracellular vesicle EV from endothelial cells EC called exosomes Therefore the development of a highly sensitive reproducible and easy to implement assay to detect exosome concentrations in complex clinical samples can have potential diagnostic and therapeutic benefit In this research proposal we will develop and characterize a novel Magnetic Exosomal Assay kit and reader for fast isolation and detection of single intact exosome concentrations in human serum In addition we will assess the optimized Magnetic Exosomal Assay on Community Acquired Pneumonia patient serum The ultimate goal of this research is to develop a highly sensitive reproducible and easy to implement exosomal assay as a diagnostic tool for patients with various types of impaired vascular integrity related diseases PUBLIC HEALTH RELEVANCE This Small Business Innovation Research Phase project aims to develop and characterize a novel Magnetic Exosomal Assay to isolate and detect single intact exosome concentrations in human serum Successful completion of the project will advance diagnosis and prognosis for patients with various types of impaired vascular integrity related diseases which often disproportionally affect women the elderly and disadvantaged and or underrepresented races and or ethnicities