High-throughput single-cell sorting and kinetic analysis of secreted particles

DESCRIPTION provided by applicant Advances in reagents and analytical tools have improved characterization of cellular phenotypes However most studies are still performed on cell populations that are morphologically and genetically homogeneous yet have important biological heterogeneity with individual cells having unique phenotypic or `omic profiles This leads to an average measurement of individual cells none of which may actually be average Single cell analysis can define the scope of cell to cell variations within a population and provid in depth analysis of single exceptional cells in contexts such as stem cell differentiation or cancer where these cells can give rise to clonal populations that drive the biology While novel tools such as multiparameter flow cytometry mass cytometry and image based cytometry provide in depth analysis of individual cells these technologies only provide a single time point andquot snapshotandquot and lack downstream molecular analysis This project will develop a integrated cell sorting and single cell positioning device to allow single cells to be tracked and measured over time This system will use a conventional multi well plate format allowing the use of existing microscopy and analysis platforms PUBLIC HEALTH RELEVANCE The advances proposed here will allow NanoCellect to achieve its mission to advance single cell technology that conforms to standard multiwell plate platforms This will be achieved by integrating a microfluidic cell sorter and silicon based plate well insert that can arrange thousands of cells into hydrophilic zones each containing a single cell