New bioassay method for the measurement of polyphenol-induced immune activation

DESCRIPTION provided by applicant Herbal nutritional supplements are widely consumed by the public for their immune health properties The most andquot acceptedandquot method for analyzing these products that purportedly reflects their immune health properties is the andquot ORACandquot value which is a measure of total anti oxidant activity Though anti oxidant activity is thought to have health benefits including effects on the immune system there is very little evidence of this benefit in vivo Polyphenols are one of the major classes of compounds found in many nutritional supplements with anti oxidant activity We found that a subset of polyphenols oligomeric procyanidins OPCs possess a unique capacity to enhance the immune system distinct from anti oxidant activity Specifically some polyphenols induce rapid induction of key immune cytokines that are critical to immune responses Induction is due to enhanced transcriptional activity as well as enhanced transcript mRNA stability This latter activity is absolutely unique We developed a cell based reporter assay for this activity which we propose as a new screening tool for rating the immune enhancing effects of nutritional supplements This application application aims to improve upon this prototype bioassay by increasing the cell line robustness and identifying control standards These studies will generate an optimized product for screening the immune activity of polyphenol containing nutritional supplements PUBLIC HEALTH RELEVANCE Nutritional supplements are commonly used as medicines worldwide Many are prescribed for their use in modulating the immune response However currently available assays are very poor predictors of the immune systemandapos s response to nutritional supplements This project aims to develop a new class of bioassay to address this limitation by directly measuring how a select class of polyphenols oligomeric procyanidins promote immune responses