A Multiplexed Assay for the Detection of Pathogens of Military Importance in Sand Flies

The objective of this study is to develop a robust, field grade, screening assay for the detection of both leishmania parasites and/or sand fly fever viruses directly in sand flies. The test will utilize a multi-line lateral flow (dipstick) chromatography incorporated into a cassette that enables the simultaneous screening of both analytes. The proposed immunochromatographic assay would be a very useful screening test to assess sand fly infestations for leishmania parasites or sand fly fever viruses. This would facilitate the treatment of military personnel presenting with febrile illness. It would also help in eradication programs. In this Phase I study we will develop/acquire antibody and antigen reagents for use in development of prototype antigen detection assays for both pathogens. We will then construct prototype assays that will be evaluated against archived samples from infected sandflies. Further development of the assay would take place in a Phase II proposal.