Development of an Advanced Fluidic Sample Bioprocessor

This proposal describes a completely automated, NanoBioSentinel sample preparation instrument for biodefense. The NanoBioSentinel will contain two modules: a bead-based Sample Capture and Purification Module (SCPM) front-end and a microchip-based NanoBioProcessor Module. Built partly upon existing devices, the SCPM will use immunocapture and multi-dimensional purifications to process milliliter volumes into microliter volumes. Target organisms and agents are captured, concentrated, and purified on antibody-conjugated beads before lysis followed by bead-based nucleic acid binding and purification in the SCPM. Purified samples on beads are then moved into the NanoBioProcessor Module for fluidic sample processing in a microchip format. The NanoBioProcessor microchips have simple on-chip valves, pumps, and routers and can perform most chemistries. In Phase I, the sample preparation method to be evaluated will be sample preparation for a two-dimensional detection: (1) microscale-Real Time-PCR and (2) microchannel capillary array electrophoresis fragment sizing for confirmation of positives. While we develop the ¿backend¿ targeting these chemistries, the NanoBioSentinel will be able perform most chemistries and feed most analytical platforms. The technology will be the basis of sample preparation and analysis systems for pathogen detection, diagnostics and biodefense.