A Novel Electrokinetics-Driven, Integrated Microfluidic Cartridge for Sample Preparation from Complex Matrices

Sample preparation has been recognized as the single most important challenge to be faced in the development of detect-to-warn (DTW) systems (NRC, 2005). Available commercial sample preparation technologies are expensive, slow (30 min to 2 hours) and require trained laboratory technicians for operation. Addressing this need, we propose to design, fabricate, and demonstrate a novel integrated microfluidic cartridge exploiting advanced electrokinetics for preparation of complex liquid samples for genomic analysis. Building on CFDRC¿s expertise and proprietary platform in Phase I, we will demonstrate separation of bacterial cells from a Contaminant-laden solution, followed by reagent-less lysis to extract the genomic content. Device designs will be guided by proven high-fidelity simulation methods developed by CFDRC. The microfabricated components will be tested and demonstrated in our well-equipped microfluidics laboratory. In Phase II, an integrated microfluidics sample preparation cartridge will be developed for ready interfacing with existing/next-generation detection technologies. The integrated cartridge will consist of separation, lysis, purification, pre-concentration and COTS components. Phase II prototype will be extensively demonstrated and characterized for samples containing various genomic targets in the presence of common atmospheric background (dust, pollen, etc.).