A NOVEL IMMOBILIZED ENZYME ELECTRODE

THIS RESEARCH IS DIRECTED TOWARD THE DEVELOPMENT OF IMPROVEDIMMOBILIZED ENZYME BIOSENSOR TECHNOLOGY. IMMOBILIZED ENZYMEBIOSENSORS ARE IMPORTANT COMPONENTS OF IN VIVO MONITORING AND CLINICAL ANALYSIS SYSTEMS. TO DATE, THE MAJOR PROBLEMS WITH THESE BIOSENSORS ARE INSTABILITY DUE TO POOR ENZYME IMMOBILIZATION TECHNIQUES AND LIMITED SENSITIVITY TO AMPEROMETRIC DETECTION OF PEROXIDE, THE REACTION PRODUCT DETECTED BY THE SENSOR. IN PHASE I, IT IS PLANNED TO DEMONSTRATE TWO SEPARATE INNOVATIONS ADDRESSING THESE TWO PROBLEMS: (1) A NOVEL ENZYME IMMOBILIZATION PROCEDURE, AND (2) A NOVEL METHOD FOR QUANTITATIVE PEROXIDE DETECTION. TAKEN TOGETHER, THESE INNOVATIONS HAVE THE POTENTIAL TO SIGNIFICANTLY IMPACT BIOSENSOR TECHNOLOGY FOR A VARIETY OF APPLICATIONS. PHASE I EFFORTS ARE DIRECTED TOWARD THE DEVELOPMENT OF A GLUCOSE OXIDASE BIOSENSOR FOR GLUCOSE DETECTION, INCORPORATING THESE INNOVATIONS. HOWEVER, THESE INNOVATIONSARE WIDELY APPLICABLE TO OTHER IMMOBILIZED ENZYME AND IMMOBILIZED ENZYME-MEDIATOR BIOSENSORS.