Novel wedge-based approach for simultaneous multichannel microscopy

DESCRIPTION (provided by applicant): Physical Sciences Inc. (PSI) and the laboratory of Christopher Gabel at Boston University School of Medicine propose to design, develop, characterize, and demonstrate a novel approach to simultaneous multichannel microscopy that will overcome a number of limitations of existing technologies. Simultaneous multichannel microscopy techniques measure changes in multiple observables that are reported by different wavelengths of light, and these techniques are finding greaterapplication in many areas of biological research as noninvasive measurements become more attainable and desirable. Example applications include in vivo ratiometric neuronal calcium imaging, co-localization of multiple fluorescent proteins and labels, and non-invasive pH measurement. Despite the ubiquity of multichannel imaging and the existence of three current commercial approaches to perform simultaneous multichannel imaging, limitations to each approach prevent full utilization and exploitation of t