ELECTROSPUN BIODEGRADABLE MEMBRANE INSERTS (POP:7/27/2020 TO 04/26/2021)

The need for more complex 3D tissue models by pharmaceutical and academic researchers is increasing. A key factor for certain tissue models is a scaffold that promotes structural integrity, cell anchorage, viability and proliferation but which over time degrades (so that it can be replaced by naturally produced ECM) and allows cell-to-cell interaction in co-culture models. No high throughput, cell culture insert plates (CCIP) are currently available with biodegradable membranes. In the current proposal, we will produce a 96-well CCIP with a biodegradable membrane. During Phase 1, a biocompatible, electrospun membrane of the prescribed biodegradation rate, thickness, and pore size will be identified. Initially, we will test biocompatible adhesives and develop techniques to attach the membrane to single-well cell culture inserts (CCI) along with a ridge that will be attached to the CCI underside. CCI biocompatibility will be verified by culturing a highly differentiated, 3D tissue model for ≥30 days. The techniques to adhere the membrane and ridge will be adapted to produce a prototype 96-well CCIP and its properties will be verified. In Phase 2, molds for the CCIP and accompanying plates will be made, and membrane and ridge adhering manufacturing techniques will be refined to allow commercial production of 96-well CCIP.