Novel Raman Probes for Intracellular Spectroscopy of Signaling Pathways (PSI-7265-230)

79728S The real-time intracellular monitoring of signaling pathways represents the next step in the elucidation and control of complex biological processes at the molecular level. Contrast agents or probes are required for molecular targeting and visualization of individual signaling events. Metallic nanostructures that produce surface enhanced Raman scattering (SERS) come closest to being the ideal intracellular probe. In previous work, a reproducible metallic SERS nanoprobe has been developed, and an increase in the Raman cross-section of cytochrome c (cyt-c), the key signaling protein in apoptosis, was demonstrated. This project will use the metallic nanoprobes to demonstrate the real-time detection of cyt-c release in viable cells that are stimulated to enter apoptosis. Phase I will demonstrate the delivery of multiple intact nanoprobes into cells with retention of cell viability and apoptotic function. These probe-loaded cells will be stimulated to enter apoptosis, and the temporal profile of cyt-c concentration will be measured. Commercial Applications and Other Benefits as described by the awardee: The nanoprobes should have the potential to elucidate intracellular signaling pathways at an unprecedented level of detail, providing capabilities for military medicine, civilian medicine, drug discovery, and clinical diagnostics.