Formulation of TLR 3, 7, and 8 Agonists in Conjugatable Adjuvant Lipid Vesicles

DESCRIPTION (provided by applicant): The overall goal for this SHIFT SBIR application is to develop new, easy-to-use conjugatable adjuvant lipid vesicle (CALV) kits which contain Toll-like Receptor (TLR) agonists 3 and 7/8. Utilizing our proprietary VesiVax(R) CALV system and the Bacillus anthracis antigens, Protective Antigen (PA) and poly-3-D-glutamic acid (PGA), as model antigens, we will evaluate the adjuvant effect stimulated by candidate TLR3 and TLR7/8 agonists. We have already shown that the TLR4 agonist, MPL has an immunostimulatory effect and hypothesize that other TLR agonists will influence the immune response profile elicited to a particular target antigen and can be correlated with protective immunity against various pathogens. The rationale for testing liposomes containing different TLR agonists is to dissect the adjuvant effect of the TLR pathways by activating one or more of the receptors, and produce the optimal immune response for a target antigen of interest. We will evaluate in vitro andin vivo the immune responses elicited by the different TLR3 and TLR7/8 agonist formulations to our model antigens by characterizing antibody and cytokine production. The overall goal is to create an optimized set of kits that can be easily used by vaccinologists and immunologists to generate an effective immune response against the target antigen. PUBLIC HEALTH RELEVANCE: With the continued emergence of new pathogens, and the threat of bioterrorism, it is imperative to continue to develop methods and reagents for immunologists and vaccinologists in order to rapidly generate vaccines to particular antigen(s) of interest. The VesiVax(R) vaccine system was designed to be easily manipulated, so that target antigens could be displayed from the surface ofthe immunogenic liposomes for subsequent use in immunization studies. Many antigens of interest are already available as proteins, peptides or carbohydrates, therefore, we expanded the VesiVax(R) system to create conjugatable adjuvant lipid vesicles (CALV) that allow for direct conjugation of antigens to liposomes, as well as the incorporation of adjuvants like the Toll-like Receptor (TLR) agonists. Easy-to-use CALV kits containing TLR3 and TLR7/8 agonists will be developed through this SHIFT SBIR application for sale to vaccinologists and immunologists for use in research. The development of the VesiVax(R)-TLR CALV kits for use as an adjuvant will greatly increase the chances of developing effective vaccines that will ultimately be of great benefit to public health.