Self-Contained Dual-Mode Sample Preparation Module Using Non-Hazardous Reagents

AI Biosciences proposes to demonstrate the ability of a microgravity-compatible, compact, dual-mode automated sample preparation cartridge (DM-SPC) platform that processes samples from various sample matrices (swab, potable water, blood, urine, etc.) to yield high-quality nucleic acids for downstream molecular detection and identification in a closed-cartridge system. The use of novel, non-hazardous reagents will ensure crew safety. Its primary function will enable NASA to rapidly identify microorganisms that could affect crew safety. The design allow both manual and automated operation of the process. This highly flexible system, when operated in auto-mode, will allow previously complicated, labor-intensive, and time-consuming processes to be carried out by a programmable, turn-key, and closed system using pre-filled cartridges. The DM-SPC can also be used to capture and purify cell and protein targets.