Self-Contained Dual-Mode Sample Preparation Module Using Non-Hazardous Reagents

Building on the successful function test demonstrated in Phase I, including a parabolic flight test, AI Biosciences proposes to further advance the capability of a microgravity-compatible, compact, dual-mode sample preparation module (DM-SPM) in Phase II. This module processes samples from various matrices—such as swabs, potable water, blood, and urine—to yield high-quality nucleic acids for downstream molecular detection and identification within a closed-cartridge system. Our technology can fill NASA's technology gap in sample preparation in space. Utilizing novel, non-hazardous reagents inside a dual-mode sample preparation cartridge (DM-SPC) will enhance crew safety. Its primary function will enable NASA to quickly identify microorganisms that could compromise crew safety. The system can also be extended to study the microbiomes of the space station and future space outposts, observing changes over time. This near-term deployable cartridge and platform system will additionally have the capability to perform other magnetic particle-based applications. This highly flexible system will allow previously complicated, labor-intensive, and time-consuming processes to be executed by a programmable, turn-key, closed system using pre-filled cartridges. Furthermore, the SPM can be employed to capture and purify cell and protein targets. The self-contained DM-SPC is also gravity and reagent-agnostic when paramagnetic magnetic particle-based protocols are followed. At the end of Phase II, a fully automated platform will be tested for nucleic acid extraction during parabolic flights.