Single cell tri-omics: High coverage simultaneous profiling of DNA, RNA, and proteins in single cells

ABSTRACT Alzheimer's disease, a devastating neurologic disorder, accounts for 60-80% of all dementias, making the development of new technologies for studying the disease crucial. Fluid Discovery aims to advance single-cell proteomics with a groundbreaking product called Self-Assembled Partitioning Proteomics (SAP-pm), which will quantify thousands of proteins in hundreds of thousands of individual cells and can be multiplexed with single cell DNA and RNA sequencing. Building on cutting-edge work in microfluidics, biophysics, and genomics, SAP- pm offers a unique, rapid, and cost-effective method to overcome limitations in protein coverage and sensitivity found in current technologies. The project comprises two aims: (1) Develop SAP-pm for sensitive, thousand-plex analysis of proteins at the single cell level, and (2) Expand the capability of SAP-pm to analyze brain nuclei and simultaneously capture mRNA, facilitating valuable insights from primary human tissues to improve human health.