Fluorescent Qdot-Antibody or Aptamer Test Strips for Ultrasensitive Algal Toxin Detection

Nanohmics, Inc. proposes to develop the most sensitive antibody and/or DNA aptamer‐quantum dot (Qdot)‐based lateral flow (LF) test strips possible for detection of harmful algal bloom (HAB) toxins in fresh or saltwater and seafood rinsates. Although commercial colloidal gold‐LF test strips for some HABs and anti‐HAB toxin aptamer DNA sequences already exist, Nanohmics’ innovation of incorporating aptamers and fluorescent Qdots into LF test strips will significantly improve sensitivity. In addition, multiple line LF strips can be constructed with multiple colored fluorescent emissions based on Qdot size (multiplexing) with one common UV excitation source such as a simple mineral light. In addition to visual assessment, the LF test strips will be quantified via a smart phone camera adaptor to make fieldbased quantitation of algal toxins simple and easy with rapid sharing of data via the internet and cloud storage. In Phase II, Nanohmics will expand its menu of HAB toxin assays and complete its smart phone sensor integration. Nanohmics will then deliver a prototype system to NOAA for testing and evaluation and either manufacture the sensors and LF test strips itself or license the technology to companies already in the algal toxin assay space.