Developing Silicon Nanowire Sensors to Measuring Host-Cell Proteins at a Biomanufacturing Line

Our biosensor is made of silicon nanowire arrays functionalized with antibodies to measure the concentration of specific proteins. In this grant we will take a sensor that has been demonstrated for use in measuring a set of generic host cell proteins and we will apply the sensor to measure a set of proteins for a specific cell line. In doing so, we will demonstrate the utility of our sensor to be used as a cell line-specific platform for biomanufacturers who require custom sensors to measure host cell proteins for their specific cell lines. We will then make a portable system, including both the hardware and software, and bring it to a biomanufacturer’s line to do measurements side by side with ELISA tests. Lastly, we will teach their staff to run measurements using our sensors and system and use their feedback to write a manual for use.