A 3D Printer Enabled, High Performing, Microgravity Compatible, and Versatile Sample Preparation Platform

AI Biosciences proposes to demonstrate the ability of a microgravity-compatible, compact, light-weight, and automated versatile sample preparation platform (VSPP) that processes samples from various sample matrices (swab, potable water, blood, urine, etc.) to yield high quality nucleic acids for downstream molecular detection and identification in a closed-cartridge system. Its primary function will enable NASA to rapidly identify microorganisms that could affect crew safety. This near-term deployable cartridge and platform system also has the option to perform isothermal and PCR amplification of nucleic acids. This highly flexible system will allow previously complicated, labor-intensive, and time consuming processes to be carried out by a turn-key and closed system using pre-filled cartridges. We have demonstrated on the ground in a laboratory setting that the entire setup for high quality DNA/RNA extraction from urine, whole blood, serum, and saliva can be achieved via simple and reversible retrofitting of a low-cost fused deposition modeling (FDM) based 3D printers ranging from $199 to $750. The VSPP can also be used to capture and purify cell and protein targets. The ability of having a dual-use 3D printer in-flight to carry out sample preparation and additive manufacturing in-flight can be an attractive option in long duration space flights.