Yeast Based Biosensor for Oganophosphate Detection

There exists a military and homeland security need for rapid, accurate and sensitive methods for the detection of organophosphates in cluttered environments. The in vitro modification and selection (IVMS) process under development by the Luna teamovercomes the limitations of current biosensor technologies by converting a known biomolecular sensor - the receptor for a steroid hormone - into a new sensor that specifically recognizes the target organic compound of choice.For this program, Luna Innovations and the University of Virginia are utilizing the IVMS process to develop a flexible biosensor platform for the detection of VX. During the Phase I program, the Luna team has focused on developing the receptor technologyand demonstrating its feasibility for the detection of organophosphates in a yeast-based biosensor. Specific Phase I program achievements include the optimization of the screening process, selection of a receptor library enriched for receptors that bindDemeton-S, and demonstration of a yeast-based FRET biosensor technology with ppb sensitivity. During the Phase II program, Luna will leverage the experience with Demeton-S to develop a rapid, reliable, and sensitive detection system for VX, easilyexpandable to incorporate additional organic chemical targets of interest. Research concerning biosensors will yield immediate applications in 1) chemical/ biological agent detection, 2) mine and explosives detection, 3) drinking and wastewater monitoring, 4) therapeutic drug monitoring, 5) chemical analysis, and 6) intelligentprocess monitoring of advanced materials. The technology demonstrated during the Phase I program and proposed for continued development in Phase II will allow Luna to capture a significant share of the initial target market segment through Luna's provenability to transfer products from research to market.