Microfluidic Real-Time Monitoring Array with Graphene Enhanced Impedance Sensing for Coagulation Biomarker Profiling

About 40% of trauma-related deaths are related to rapid onset of coagulopathy and the vast majority of trauma patients are coagulopathic at the time of death. Lynntech proposes to develop a novel, easy-to-use and cost effective device for simultaneous quantitative real-time detection of multiple proteins involved in the hemostatic mechanism, using human unprocessed whole blood as the input sample. Design features suitable for application as a point-of-care device will render it suitable for application both in the field (pre-hospital) and hospital setting. This will be accomplished using Lynntechs disposable, electrochemical micropump-fluidics and electrochemical impedance system in a disposable analysis cartridge format. Micropumps will drive the whole blood over individual sensors functionalized with capture reagents against corresponding target analytes. Changes in impedance to current flow due to binding of the target analyte to the corresponding sensor will be used to quantify analyte concentration. The operational capacity and functionality of the sensor will be optimized for multiple recordings which will allow real-time quantification of the target analytes for temporal resolution of changes in analyte concentration at physiological time scales. The goal of the Phase I research effort is to demonstrate the technical feasibility of key components of the proposed electrochemical micropump-fluidics system.