Activated Organophosphate Hydrolase for Coupling to Human Skin

This Phase II STTR proposal describes an activated form of organophosphate hydrolyzing enzyme that, when dissolved in water, forms a covalent bond to skin. In Phase I, a lyophilized preparation of Organophosphate Hydrolase (OPH) from Pseudomonas diminutaretained the capability to couple to skin and to hydrolyze nerve agent surrogates when stored for months at 40¿C. Immobilized enzyme also retained the capability to hydrolyze surrogate after washing the skin with soap and water. The activation method wasalso compatible with other organophosphate hydrolyzing enzymes, including OPAA. As a contingency, Lynntech has developed a second formulation in which the enzyme does not covalently attach to skin should the covalent attachment of enzymes to skin raiseburdensome regulatory concerns. In Phase II, Lynntech will collaborate with Texas A&M University and Battelle Memorial Institute to further develop and evaluate the technology. The Phase II research will include the optimization of the activity andstability of both formulations, demonstration of the capability of the formulations to protect against pesticide and nerve agent exposure, and showcasing of the technology to potential Phase III partners. The Phase II research will conclude with deliveryof activated enzyme to the Department of Defense for further testing and evaluation. Chlorpyrifos, sold under the trade name Dursban and Lorsban, is only one of many organophosphate pesticides in current use. The EPA has estimated that between 20million and 24 million pounds of chlorpyrifos are synthesized annually. There exits a substantial commercial potential for a reactive barrier to be used by those who handle large amounts of organophosphate pesticides. These individuals primarily consistof agricultural workers but also include those involved in the synthesis, transportation, and distribution of these chemical compounds.